jc 1 kit  (Beyotime)

Journal: Genes & Diseases

Article Title: PDK1 elevation was induced by epigenetic modifications of KDM3A and METTL16 to mediate TKI resistance and cancer development

doi: 10.1016/j.gendis.2025.101947

Figure Lengend Snippet: PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL assay was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial membrane potential (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: After different treatments for 48 h, the mitochondrial membrane potential of cells was detected with the enhanced mitochondrial membrane potential assay kit using JC-1 probe (Beyotime, Jiangsu, China), and the fluorescence was analyzed with a fluorescence microscope.

Techniques: Expressing, CCK-8 Assay, Software, Flow Cytometry, TUNEL Assay, Staining, Fluorescence, Microscopy, Membrane, Standard Deviation

Journal: Materials Today Bio

Article Title: Dual-targeted manganese-doped carbon dots activate the cGAS–STING pathway and immunogenic cell death for potent glioblastoma immunotherapy

doi: 10.1016/j.mtbio.2026.103073

Figure Lengend Snippet: Mn-CDs induce mitochondrial dysfunction and immunogenic cell death (ICD) via robust ROS generation. Intracellular ROS generation in CT2A cells treated with PBS, CDs, or Mn-CDs for 4 h. (A) Representative confocal images using DCFH-DA staining (green fluorescence). Scale bar: 50 μm. (B) Flow cytometric analysis and (C) corresponding quantitative analysis of ROS-positive cell populations (n = 3). The results indicate a surge in ROS levels in the Mn-CDs group. (D) UV–vis absorption spectra of Methylene Blue (MB) mixed with H 2 O 2 and different concentrations of Mn-CDs. The degradation of MB indicates the generation of hydroxyl radicals (·OH) via manganese-mediated Fenton-like reactions. Analysis of mitochondrial membrane potential using JC-1 staining. (G) Confocal images of CT2A cells treated with PBS, CDs (25, 50 μg/mL), or Mn-CDs (25, 50 μg/mL). Red (aggregates) and green (monomers) fluorescence represent high and low mitochondrial membrane potential, respectively. Scale bar: 100 μm. (E) Quantitative analysis of the red/green fluorescence percentage. (F) Quantification of extracellular ATP release in the culture supernatant of CT2A cells. (J) Representative confocal images and (H) quantitative analysis of CT2A cells nuclear fluorescence intensity of HMGB1. The decrease in nuclear intensity indicates the release of HMGB1 from the nucleus. Scale bar: 100 μm. (K) Representative confocal images and (I) quantitative analysis of CRT exposure on the surface of CT2A cells. Scale bar: 100 μm. Transmission electron microscopy (TEM) images showing mitochondrial ultrastructure in (L) CT2A and (M) GL261 cells treated with PBS, CDs, or Mn-CDs. Scale bars: 2 μm (low magnification) and 500 nm (high magnification inset). Mn-CDs treatment induces mitochondrial swelling and cristae disruption. Data are presented as mean ± SD (∗∗∗∗p < 0.0001; ns: no significance).

Article Snippet: The JC-1 Assay Kit, DAPI, Mito-Tracker Green, Crystal Violet Staining Solution, and Protease/Phosphatase Inhibitor Cocktail (P1045) were purchased from Beyotime Biotechnology (Shanghai, China).

Techniques: Staining, Fluorescence, Membrane, Transmission Assay, Electron Microscopy, Disruption

Journal: Materials Today Bio

Article Title: Ultrasmall Prussian blue–integrated cryogel for enhanced ROS scavenging and immunomodulation via cGAS–STING inhibition in wound healing

doi: 10.1016/j.mtbio.2026.103056

Figure Lengend Snippet: Schematic representation of the synthesis process and therapeutic properties of Cryogel@USPB applied in wounds. (A) Fabrication process of Cryogel@USPB. (B) Cryogel@USPB promotes acute/chronic wounds through regulating macrophage polarization and restraining mitochondrial dysfunction via the cGAS-STING pathway.

Article Snippet: The Catalase Assay Kit and Mitochondrial Membrane Potential Assay Kit (JC-1) were from Beyotime Biotechnology Inc. (Shanghai, China).

Techniques:

Journal: Materials Today Bio

Article Title: Ultrasmall Prussian blue–integrated cryogel for enhanced ROS scavenging and immunomodulation via cGAS–STING inhibition in wound healing

doi: 10.1016/j.mtbio.2026.103056

Figure Lengend Snippet: The biocompatibility and antioxidant capability assessment of Cryogel@USPB. (A) Cell viability of NIH 3T3 and Raw264.7 cells after co-incubation with Cryogel@USPB at various concentrations (n = 3). (B) Representative fluorescent staining images of live (green)/dead (red) cells of Raw264.7 cells following co-incubation with Cryogel@USPB. (C) Determination of H 2 O 2 depletion capability of Cryogel@USPB (n = 3). (D) SOD-like activity and (E) POD-like activity of Cryogel@USPB (n = 3). (F) ·OH depletion capability and (G) •O 2 − depletion activity using EPR. (H) Representative fluorescent staining images of intracellular ROS in Raw264.7 cells following the treatment of H 2 O 2 and Cryogel@USPB. (I) TEM images of the mitochondria in RAW264.7 cells. (J) Fluorescent images of mitochondrial membrane potential in cells following different treatments. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The Catalase Assay Kit and Mitochondrial Membrane Potential Assay Kit (JC-1) were from Beyotime Biotechnology Inc. (Shanghai, China).

Techniques: Incubation, Staining, Activity Assay, Membrane